趙美萍

學術簡曆

• 2010 –至今   教授，beat365官方网站
• 2002 –2010   副教授，beat365官方网站
• 1998.7-1998.11   訪問學者 荷蘭能源研究機構 環境分析部
• 1997.9–1998.6   訪問學者 荷蘭阿姆斯特丹大學 環境與毒理化學系
• 2002   理學博士 beat365 beat365
• 1993   理學碩士 beat365 環境科學中心
• 1990   理學學士 beat365 技術物理系

科研領域及興趣

本課題組緻力于探索和構建新穎的分子識别體系，發展實時、在線的熒光分析方法，為生命科學研究、臨床檢驗、環境監測和食品安全監控提供高效的分析測量手段。目前的主要研究方向：

1. 核酸酶與核酸相互作用的分子機理研究；
2. 活細胞的不同亞細胞區室内核酸修複蛋白的原位熒光成像和結構解析；
3. 基因損傷、基因突變、多重微生物基因序列的超高靈敏檢測；
4. 多聚蛋白分子印迹識别材料的制備方法和應用。

代表性論文

1. XJ Cao, JH Zheng, RL Zhang, Y Sun, MP Zhao*, Live-cell imaging of human apurinic/apyrimidinic endonuclease 1 in the nucleus and nucleolus using a chaperone@DNA probe, Nucleic Acids Research, 2024, 52(8), e41.
2. W Chen,HQ Xu, SB Dai, JY Wang, ZY Yang, YW Jin, MB Zou, XJ Xiao*, TB Wu*, W Yan, B Zhang, ZM Lin, MP Zhao*, Detection of low-frequency mutations in clinical samples by increasing mutation abundance via the excision of wild-type sequences, Nature Biomedical Engineering, 2023, 7, 1602. (https://rdcu.be/dhUlL).
3. ZY Yang, W Chen, JY Wang, MH Shi, RL Zhang, SB Dai, TB Wu*, MP Zhao*, Programmable One-Pot Enzymatic Reaction for Direct Fluorescence Detection of Ultralow-Abundance Mutations in the DNA Duplex, Analytical Chemistry, 2021, 93, 7086.
4. JQ Zhai, MH Zhao, XJ Cao, MY Li*, MP Zhao*, Metal-ion-responsive bionanocomposite for selective and reversible enzyme inhibition, Journal of the American Chemical Society, 2018, 140, 16925.
5. TB Wu, YF Yang, W Chen, JY Wang, ZY Yang, SL Wang, XJ Xiao, MY Li, MP Zhao*, Noncanonical substrate preference of lambda exonuclease for 5'-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction, Nucleic Acids Research, 2018, 46, 3119.
6. TB Wu, W Chen, ZY Yang, HC Tan, JY Wang, XJ Xiao, MY Li, MP Zhao*, DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA, Nucleic Acids Research, 2018, 46(4), e24.
7. XJ Xiao, TB Wu, L Xu, W Chen, MP Zhao*, A branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations, Nucleic Acids Research, 2017, 45 (10): e90.
8. JQ Zhai, YB Liu, S Huang, SM Fang, MP Zhao*, A specific DNA-nanoprobe for optically tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells, Nucleic Acids Research, 2017, 45(6), e45.
9. XJ Xiao, TB Wu, FD Gu, MP Zhao*, Generation of artificial sequence-specific nucleases via preassembled inert-template, Chemical Science, 2016, 7, 2051.
10. SM Fang, L Chen, MP Zhao*, Unimolecular chemically modified DNA fluorescent probe for one-step quantitative measurement of the activity of human apurinic/ apyrimidinic endonuclease 1 in biological samples, Analytical Chemistry, 2015, 87, 11952.
11. ZY Chen, L Xu, Y Liang, MP Zhao*, pH-Sensitive water-soluble nanospheric imprinted hydrogels prepared as horseradish peroxidase mimetic enzyme, Advanced Materials, 2010, 22, 1488.
12. C Song, MP Zhao*, Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with  exonuclease cleavage, Analytical Chemistry, 2009, 81, 1383.